APPLICATION NOTE
Automation of the CRISPR-based cell line development workflow
Targeted gene editing, especially with CRISPR, has revolutionized science and has many applications in various fields, mainly in cell line development, cell and gene therapy.
An increase in demands for faster high-throughput cell-line development and better screening in the pharmaceutical space is driving research facilities and companies to automate existing labs.
Typical cell-line development requires the screening of tens of thousands of clones to find the best stable cells that yield high amounts of bioproducts. In this application note, we discuss the automation of a mammalian cell-line development workflow—the automated, integrated screening of transfected cells for edits, monoclonality, and growth assessment.