Triple negative breast cancer is a clinically aggressive tumor subtype with high rates of metastasis, recurrence, and drug resistance.

Currently, there are no clinically approved small molecule targeted therapies for this disease, which underlines the critical need to discover new therapeutic targets.

In this application note, we describe automated imaging and cell culture methods to scale up complex 3D cell-based assays. Additionally, we share advanced analysis approaches that allow scientists to gain more information about complex cellular systems, disease phenotypes, and compound effects.

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