Stem Cell Research and Presentations
Explore solutions for the complexities that come with stem cell data analysis and visualization.
ISSCR On-Demand
At ISSCR 2021, attendees discovered how new technologies in high-content imaging are revealing significant data from complex biological systems that can help reduce lab costs and push the boundaries of their research.
Presentations emphasized the prominence automation plays in ensuring organoid cultures are consistent and reproducible in complex workflows, and shared methods to simplify multivariate analysis and multi-parametric characterization.
In case you didn’t get a chance to attend, you can check out the highlights below.
INNOVATION SHOWCASE: Automated Culture and High-Content Imaging of 3D Lung and Cardiac Organoids for In Vitro Assessment of Compound Effects
Presenter:
Oksana Sirenko, Ph.D. – Sr. Scientist, Molecular Devices
Stem cell-derived and 3D cell models are becoming increasingly popular for studying complex biological effects, tissue functionality, and diseases. While complex cell models remain a hurdle for the wider adoption in research and drug development, industry leaders are removing barriers with innovative hardware and software solutions that automate cell culture, monitoring, and cell analysis. Simultaneously, advances in high-content imaging technology are helping to reveal significant information from these complex biological systems, inviting scientists to push the boundaries of their research. In this webinar, we describe an integrated workflow that allows us to automate processes of cell culture, imaging, and cell maintenance to monitor the development of stem cells and organoids, as well as characterize the complex responses to test compounds. The method was applied for automation of three complex workflows: iPSC maintenance, lung toxicity assay using 3D organoids, and monitoring compound effects on functional activity in 3D cardiac cultures.
PRESENTATION - Monitoring organoid development and characterization of calcium oscillation activities in iPSC-derived 3D cerebral organoids
Presenter:
Angeline Lim, Ph.D. - Applications Scientist, Molecular Devices
Learn how successful monitoring of cerebral organoids can be achieved with AI-based analysis tools which enables robust high-throughput analysis. The poster also outlines:
- The presence of calcium flux and the organization of the cells within the organoid is consistent with in vivo cortical development
- Our preliminary results highlight the potential use of cerebral organoids for future compound screening assays
PRESENTATION - Deep learning-based image analysis for label-free live monitoring of iPSC 3D organoid cultures
Presenters:
Angeline Lim, Ph.D. - Applications Scientist, Molecular Devices
In this presentation, we discuss how artificial intelligence (AI)-based approaches to the analysis of complex images and datasets offer highly robust results for high-content imaging workflows. Further, we demonstrate how:
- Our user-friendly SINAP tool creates custom deep learning models for robust segmentation of label-free biological models
- Phenoglyphs enables intuitive machine learning-based phenotypic profiling that can be applied to complex datasets such as patient derived spheroids
- To leverage IN Carta® Image Analysis Software as an end-to-end solution, bridging the gap between complex biological questions and AI-driven answers
POSTER - Organoids for disease modeling and in vitro drug screening: Automated culture monitoring, imaging, and analysis of complex biological systems
Presenter:
Oksana Sirenko, Ph.D. – Sr. Scientist, Molecular Devices
This poster describes an automated integrated system that allows automated monitoring, maintenance, and characterization of growth and differentiation of stem cells and organoids, as well as testing the effects of various compounds. It demonstrates methods for successful automation of three complex workflows: iPSC culture, 3D lung organoids, and intestinal organoids
POSTER - High-throughput assessment of compound-induced pro-arrhythmic effects in human IPSC-derived cardiomyocytes
Presenter:
Carole Crittenden - Sr. Applications Scientist, Molecular Devices
Multiparametric assessment of various phenotypic effects allows better evaluation of potential toxicity and provides additional information about potential mechanisms of toxicity. In this poster, we outline methods for high-throughput evaluation of cardiotoxicity effects of various compounds using high-throughput cellular screening and shares methods developed for cytotoxicity evaluation and changes in cytoskeleton using high-content imaging.
Ready to get started?
We’re here to help you select the right solution for your research. Fill out the form and let’s get started!
