Axon

THE AXON GUIDE

Electrophysiology and Biophysics Laboratory Techniques

The Axon Guide Fifth Edition

What you will find in this guide

This guide serves as an information and data resource for electrophysiologists. It covers a broad range of topics ranging from the biological basis of bioelectricity and a description of the basic experimental setup to a discussion of mechanisms of noise and data analysis.

The Axon Guide Fifth Edition is a tool benefiting both the novice and the expert electrophysiologist. Newcomers to electrophysiology will gain an appreciation of the intricacies of electrophysiological measurements and the requirements for setting up a complete recording and analysis system. For experienced electrophysiologists, we include in-depth discussions of selected topics, such as advanced methods in electrophysiology and noise.

For the fifth edition, we are very pleased to be able to offer new content in the Advanced Methods in Electrophysiology section, including advanced methods developed for ion channel recording from liposomes, planar bilayers, and bacterial spheroplasts; current recording from solid-state nanopores, and the use of nanopipettes in intracellular recording.

Table of contents

  • 1. Bioelectricity
  • 2 The Laboratory Setup
  • 3. Instrumentation for Measuring Bioelectric Signals from Cells
  • 4. Microelectrodes and Micropipettes
  • 5. Advanced Methods in Electrophysiology
  • 6. Signal Conditioning and Signal Conditioners
  • 7. Transducers
  • 8. Laboratory Computer Issues and Considerations
  • 9. Acquisition Hardware
  • 10. Data Analysis
  • 11. Noise in Electrophysiological Measurements
  • Appendix A: Guide to Interpreting Specifications

[Click to view sample]

Bioelectricity
Electric Circuit
Glass Microelectrodes
Aperture Formation in Plastic cup
Pre-Treatment of Muscle Cells

THE AXON GUIDE

The most comprehensive laboratory techniques workbook—with over 250 pages—to support electrophysical and biophysical research

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